Draq7 apoptosis and cancer
Data were always exported as events per well. Collectively, these limitations hinder the depth and accuracy of collected data while burdening the investigator with labour-intensive protocols. Cell Death Differ ; 15 : — Help us improve our products. Biochemistry ; 32 : — Data analysis was performed analogically to conditions shown on A. Luna-VargasJesse D. In search for the non-invasive fluorescent probes capable of long-term monitoring of cell death in real-time, we developed and evaluated a new anthracycline derivative, DRAQ7.
Most cell death assays rely on detection of the specific marker of cell demise at . kinetic fingerprints of anti-cancer drug action when employing DRAQ7 probe.
It proved to be non-toxic to a panel of cancer cell lines grown DRAQ7 provided a sensitive, real-time readout of cell death induced by a. Quantitative and kinetic analyses of apoptotic cell death are integral components propidium iodide, DRAQ7, SYTOX), which detect only late apoptotic events . pathway of apoptosis regulated in cancer and chemotherapy?
Cells pre-labelled with CellTrace CFSE and then treated to undergo apoptosis in the presence of Annexin V were readily detectable, and demonstrated a striking shift from green fluorescence to dual fluorescence upon apoptosis Figure 5b.
Meers P, Mealy T. Calcein-AM is a non-fluorescent cell-permeable ester that can passively enter cells. Curr Protoc Cytom. In search for non-invasive fluorescent probes capable of long-term monitoring of cell death in real-time, we evaluated a new anthracycline derivative, DRAQ7.
Cell Viability & Apoptosis Biotium
Collectively, data lend a strong support to real-time pharmacological profiling using DRAQ7 6
DATA CAPS NET NEUTRALITY RULES
|McStayCathie M. Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry.
Download references. Next, we integrated YOYO3 and Annexin V staining in high-content cell imaging for real-time kinetic detection of apoptosis. To exclude this possibility, we therefore investigated a panel of cytotoxic drugs and apoptotic inducers on human hematopoietic tumor cell lines.
DRAQ7™ Staining Solution is a far-red DNA stain which is cell impermeable and intercalates double-stranded DNA (dsDNA) of dead and permeabilized cells.
This procedure results in reproducible data and clear discrimination of populations of distinctive subpopulations see Fig.
TUNEL terminal deoxynucleotidyl transferase TdT mediated dUTP nick-end labeling is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.
Ethidium homodimer III. In this regard we provide proof-of-concept evidence that such real-time labeling procedure can provide multi-parameter and kinetic fingerprints of anti-cancer drug action. Donald Wlodkowic: zn.
Kinetic viability assays using DRAQ7 probe
Several methods have been developed to assess apoptosis, cell cycle, and cell DRAQ7™. Interaction into DNA double strands. Flow cytometry. DNA content in . Data courtesy of ES Glazer and SA Curley, MD Anderson Cancer Center. 0.
MTT assay requires cell lysis before the absorbance can be measured. It is not recommended for imaging with other visible red probes.
Because bicolor assay provides multiparameter parameter data, subsequent analysis time will depend heavily on the gating strategies employed by an investigator. Data Click here to view. We found that real-time DRAQ7 assay reported the death of cells cultured under variety of perturbation and the overall responses to cytotoxic agents and resulting pharmacological dose-response profiles were not affected by the growth of cancer cells in the presence DRAQ7.
Furthermore, as experiments must be terminated prior to analysis, flow cytometry-based Annexin V assays only provide end-point data, requiring tedious optimization for treatment, timing and harvesting.